Every byte put on the SDA line must be eight bits long, with the most significant bit (MSB) being transferred first. Each byte of data has to be followed by an acknowledge bit. The acknowledge related clock pulse is generated by the controller device. The controller device releases the SDA line (HIGH) during the acknowledge clock pulse. The device pulls down the SDA line during the 9th clock pulse, signifying an acknowledge. The device generates an acknowledge after each byte has been received.

There is one exception to the acknowledge after every byte rule. When the controller device is the receiver, it must indicate to the transmitter an end of data by not-acknowledging (negative acknowledge) the last byte clocked out of the target device. This negative acknowledge still includes the acknowledge clock pulse (generated by the controller device), but the SDA line is not pulled down.

After the START condition, the bus controller device sends a chip address. This address is seven bits long followed by an eighth bit which is a data direction bit (READ or WRITE). For the eighth bit, a 0 indicates a WRITE and a 1 indicates a READ. The second byte selects the register to which the data is written. The third byte contains data to write to the selected register. Figure 6-8 shows an example bit format of device address 110000-Bin = 60Hex.

Figure 6-8 Example Device Address

Figure 6-9 I²C Write Cycle without CRC

When READ function is to be accomplished, a WRITE function must precede the READ function as shown above.

Figure 6-10 I²C Read Cycle without CRC